Electrophoretic separation of linear and supercoiled DNA in uncoated capillaries

被引：11

作者：

de Carmejane, O ^{[1
]}

Schwinefus, JJ ^{[1
]}

Wang, SC ^{[1
]}

Morris, MD ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 849卷 / 01期

关键词：

DNA; electrophoretic separation; uncoated capillaries;

D O I：

10.1016/S0021-9673(99)00527-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report electrophoretic separation of supercoiled plasmids (2-16 kilo base pairs) and linear double-stranded DNA (0.6-23 kilo base pairs) in uncoated capillaries filled with dilute hydroxyethylcellulose. Because electroosmotic flow reverses the order of elution, long plasmids spend less time in the capillary and their bandwidths are narrower than observed in coated capillaries. However, resolution is similar to that obtained in coated capillaries, because it is governed by the distribution of unresolved topoisomers. In the presence of electroosmotic how migration of supercoiled plasmids does not follow the elastic rod model that has been observed in coated capillaries. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：267 / 276

页数：10

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