Adaptive optics two-photon excited fluorescence lifetime imaging ophthalmoscopy of exogenous fluorophores in mice

被引：27
|
作者：
Feeks, James A. [1 ,2 ]
Hunter, Jennifer J. [1 ,3 ,4 ]
机构：
[1] Univ Rochester, Ctr Visual Sci, Rochester, NY 14627 USA
[2] Univ Rochester, Inst Opt, Rochester, NY 14620 USA
[3] Univ Rochester, Flaum Eye Inst, 601 Elmwood Ave, Rochester, NY 14642 USA
[4] Univ Rochester, Dept Biomed Engn, 601 Elmwood Ave, Rochester, NY 14627 USA
来源：
BIOMEDICAL OPTICS EXPRESS | 2017年 / 8卷 / 05期
基金：
美国国家卫生研究院;
关键词：
RETINAL-PIGMENT EPITHELIUM; LIVING MOUSE EYE; LIGHT RESPONSES; GANGLION-CELLS; LIVE CELLS; AUTOFLUORESCENCE; MICROSCOPY; TIME; STANDARDS; VARIANTS;
D O I：
10.1364/BOE.8.002483
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In vivo cellular scale fluorescence lifetime imaging of the mouse retina has the potential to be a sensitive marker of retinal cell health. In this study, we demonstrate fluorescence lifetime imaging of extrinsic fluorophores using adaptive optics fluorescence lifetime imaging ophthalmoscopy (AOFLIO). We recorded AOFLIO images of inner retinal cells labeled with enhanced green fluorescent protein (EGFP) and capillaries labeled with fluorescein. We demonstrate that AOFLIO can be used to differentiate spectrally overlapping fluorophores in the retina. With further refinements, AOFLIO could be used to assess retinal health in early stages of degeneration by utilizing lifetime-based sensors or even fluorophores native to the retina. (C) 2017 Optical Society of America
引用
收藏
页码：2483 / 2495
页数：13
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