Protein Tertiary Structural Changes Visualized by Time-Resolved X-ray Solution Scattering

被引：50
|
作者：
Ahn, Sena [1 ]
Kim, Kyung Hwan [1 ]
Kim, Youngmin [1 ]
Kim, Jeongho [1 ]
Ihee, Hyotcherl [1 ]
机构：
[1] Korea Adv Inst Sci & Technol, Dept Chem, Grad Sch Nanosci & Technol, WCU,Ctr Time Resolved Diffract, Taejon 305701, South Korea
来源：
JOURNAL OF PHYSICAL CHEMISTRY B | 2009年 / 113卷 / 40期
关键词：
SMALL-ANGLE SCATTERING; REFINEMENT; DYNAMICS;
D O I：
10.1021/jp906983v
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
We obtained a solution structural model of myoglobin (Mb) formed upon the CO photolysis of MbCO by analyzing time-resolved X-ray Solution scattering data. An experiment-restrained rigid-body molecular dynamics simulation was used to find the best model whose theoretical difference scattering curve gives it satisfactory agreement with the experimental data at the time delay of 10 nus. The obtained Solution model shows structural changes similar to crystallographic models for MbCO -> Mb but also displays a noticeable difference in that the N-terminus and F helix show larger structural changes.
引用
收藏
页码：13131 / 13133
页数：3
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