Medium- and long-term in vitro conservation of olive germplasm (Olea europaea L.)

被引：11

作者：

Lambardi, M ^{[1
]}

Benelli, C ^{[1
]}

De Carlo, A ^{[1
]}

Fabbri, A ^{[1
]}

Grassi, S ^{[1
]}

Lynch, PT ^{[1
]}

机构：

[1] CNR, Ist Propagaz Specie Legnose, I-50018 Scandicci, Firenze, Italy

来源：

PROCEEDINGS OF THE FOURTH INTERNATIONAL SYMPOSIUM ON OLIVE GROWING, VOLS 1 AND 2 | 2002年 / 586期

关键词：

germplasm preservation; slow growth storage; cryopreservation; vitrification;

D O I：

10.17660/ActaHortic.2002.586.14

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The potential for the conservation of olive germplasm by both slow growth storage (4degreesC), and preservation in liquid nitrogen was examined. For medium-term storage, olive shoot cultures (cvs Leccino and Frantoio) were effectively preserved in the dark at 4degreesC for up to 8 months. With cryopreservation based long-term storage, best results were obtained with portions of embryogenic tissue (cv Canino), which exhibited post-thaw regrowth and production of somatic embryos after the application of controlled rate freezing or vitrification procedures. A maximum of 15% post-thaw survival was obtained by using the vitrification/one-step freezing procedure with shoot tips from in vitro-grown plantlets of the cv Frantoio.

引用

页码：109 / 112

页数：4

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