Olive (Olea europaea L.) in vitro multiplication

被引：2

作者：

Dutra, Leonardo Ferreira ^{[1
]}

de Oliveira, Adelson Francisco ^{[2
]}

Fraguas, Chrystiane Borges ^{[3
]}

Pasqual, Moacir ^{[4
]}

机构：

[1] Univ Fed Lavras UFLA, CNPq, BR-37200000 Lavras, MG, Brazil

[2] EPAMIG CTSM, BR-37200000 Lavras, MG, Brazil

[3] Univ Fed Lavras, Programa Posgrad Fitotecnia, Lavras, MG, Brazil

[4] Univ Fed Lavras, Dept Agr, Lavras, MG, Brazil

来源：

CIENCIA E AGROTECNOLOGIA | 2004年 / 28卷 / 01期

关键词：

In vitro culture; proliferation; nodal segment; BAP; NAA;

D O I：

10.1590/S1413-70542004000100030

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

This work had the objective to induce olive multiplication. Nodal segments from in vitro plantlets were excised and inoculated in test tubes containing MS culture medium supplemented with activated charcoal (2 g L-1), BAP (0, 1, 2 and 4 mg L-1), NAA (0; 0.01; 0.1 and 1 mg L-1), agar (6 g L-1) and pH adjusted to 5.8. The explant were maintained in growth room to 25 +/- 1 degrees C, 32 mu moles.m(-2).s(-1) light intensity and 16 hours photoperiod for 100 days. There was not shoots induction in the nodal segments. Larger length of aerial part were obtained with ANA 0.1 mg L-1 in the BAP absence. Culture medium without BAP provides larger weight of fresh matter of the aerial part.

引用

页码：220 / 223

页数：4

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