Polyalanine - a practical polypeptide mass calibration standard for matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry in positive and negative mode

被引：10

作者：

Gruendling, Till ^{[1
]}

Sauerland, Volker ^{[2
]}

Barahona, Cesar ^{[2
]}

Herz, Corinna ^{[1
]}

Nitsch, Ute ^{[1
]}

机构：

[1] BASF SE, Competence Ctr Analyt, Ludwigshafen, Germany

[2] Bruker Daltonik GmbH, Bremen, Germany

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2016年 / 30卷 / 06期

关键词：

IONS; MS;

D O I：

10.1002/rcm.7492

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RationaleMass calibration in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is currently obtained using mixtures of individual peptides. This procedure has several drawbacks, including laborious preparation, limited shelf life and unequal calibration mass spacing. Polyalanine, a simple to prepare polydisperse molar mass standard, alleviates these problems. MethodsPolyalanine is prepared by the typical protocols for biological sample analytics. ResultsPolyalanine is the first polymeric standard providing abundant signals in both, positive and negative polarity mode with the typical matrices DHB and HCCA. ConclusionsFacile MS as well as MS/MS calibration is thus enabled for the first time in both polarity modes by employing this polydisperse standard. Copyright (c) 2016 John Wiley & Sons, Ltd.

引用

页码：681 / 683

页数：3

共 50 条

[21] Matrix-assisted laser desorption ionization postsource decay mass spectrometry
Wingerath, T
Kirsch, D
Kaufmann, R
Stahl, W
Sies, H
OXIDANTS AND ANTIOXIDANTS, PT A, 1999, 299 : 390 - 408
[22] Matrix-assisted laser desorption/ionization mass spectrometry - Instrumentation and applications
Muddiman, DC
Bakhtiar, R
Hofstadler, SA
Smith, RD
JOURNAL OF CHEMICAL EDUCATION, 1997, 74 (11) : 1288 - 1292
[23] Matrix ions as internal standard for high mass accuracy matrix-assisted laser desorption/ionization mass spectrometry imaging
Treu, Axel
Roempp, Andreas
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2021, 35 (16)
[24] Sample Preparation for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Kim, Jeongkwon
MASS SPECTROMETRY LETTERS, 2015, 6 (02) : 27 - 30
[25] Matrix-assisted laser desorption ionization imaging mass spectrometry in lipidomics
Fernandez, J. A.
Ochoa, B.
Fresnedo, O.
Giralt, M. T.
Rodriguez-Puertas, R.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 401 (01) : 29 - 51
[26] Chemical reactivity in matrix-assisted laser desorption/ionization mass spectrometry
Enjalbal, C
Sauvagnat, B
Lamaty, F
Lazaro, R
Martinez, J
Mouchet, P
Roux, F
Aubagnac, JL
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1999, 13 (18) : 1775 - 1781
[27] Characterization of polyesters by matrix-assisted laser desorption ionization mass spectrometry
Univ of Pittsburgh, Pittsburgh, United States
Macromolecules, 13 (3781-3787):
[28] Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of polymers
Macha, SF
Limbach, PA
CURRENT OPINION IN SOLID STATE & MATERIALS SCIENCE, 2002, 6 (03): : 213 - 220
[29] MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF BIOPOLYMERS
HILLENKAMP, F
KARAS, M
BEAVIS, RC
CHAIT, BT
ANALYTICAL CHEMISTRY, 1991, 63 (24) : A1193 - A1202
[30] Matrix-assisted laser desorption ionization imaging mass spectrometry in lipidomics
J. A. Fernández
B. Ochoa
O. Fresnedo
M. T. Giralt
R. Rodríguez-Puertas
Analytical and Bioanalytical Chemistry, 2011, 401 : 29 - 51

← 1 2 3 4 5 →