Study of Ca2+, Mn2+ and Cu2+ binding to DNA in solution by means of IR spectroscopy

被引：74
|
作者：
Hackl, EV [1 ]
Kornilova, SV [1 ]
Kapinos, LE [1 ]
Andrushchenko, VV [1 ]
Galkin, VL [1 ]
Grigoriev, DN [1 ]
Blagoi, YP [1 ]
机构：
[1] UKRAINIAN ACAD SCI,INST LOW TEMP PHYS & ENGN,UA-310164 KHARKOV,UKRAINE
来源：
JOURNAL OF MOLECULAR STRUCTURE | 1997年 / 408卷
关键词：
IR spectroscopy; DNA; divalent metal ions; binding constant; DNA compactisation;
D O I：
10.1016/S0022-2860(96)09534-8
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The interaction of Cu2+, Mn2+ and Ca2+ ions with the DNA macromolecule was studied in aqueous solutions at different metal ion concentrations. All these ions interact with both the bases and the phosphate groups of DNA. Cu2+ ions alter the DNA B-conformation at [Cu2+] > 2 x 10(-2) M. Metal ions binding to DNA induce DNA compactisation. The highly positive cooperativity of this process was shown. The binding constants and parameters of cooperativity of the metal ions binding to DNA were estimated. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：229 / 232
页数：4
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