Evaluation of mutagenicity of wastewater in the vicinity of pesticide industry

被引：19

作者：

Anjum, Reshma ^{[1
]}

Malik, Abdul ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Fac Agr Sci, Dept Agr Microbiol, Aligarh 202002, Uttar Pradesh, India

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2013年 / 35卷 / 02期

关键词：

Salmonella mutagenicity test; Industrial wastewater; DNA repair defective mutants; Genotoxicity; ORGANOCHLORINE PESTICIDES; CONTAMINATED SOIL; GENOTOXICITY; SURFACE; RIVER; POLLUTION; EXTRACTS; NORTHERN; YAMUNA; HCH;

D O I：

10.1016/j.etap.2012.12.013

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Pesticide industrial wastewater samples were taken from the Chinhat industrial area nearby Lucknow city, India. GC-MS analysis revealed the presence of pesticides lindane, alpha-endosulfan, beta-endosulfan, chlorpyriphos, monocrotophos, dimethoate and malathion. A pesticide mixture and wastewater extracts were studied to determine the mutagenicity by Ames Salmonella test, survival of DNA repair defective E. coli K-12 mutants and bacteriophage x systems. Wastewater samples were concentrated with XAD-resins as an adsorbent and liquid-liquid extraction procedure. The XAD concentrated sample exhibited maximum mutagenic activity in comparison to liquid-liquid extracted sample. TA98 strain was the most responsive strain for both test samples with (+S9) and without (-S9) metabolic activation, while other strains exhibited weak response. A significant decline of DNA repair defective E. coli K-12 mutants, bacteriophage x was observed with test samples in the survival. The intracellular damage was highest when treated with XAD concentrated sample as compared to liquid-liquid extract after 6h treatment. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：284 / 291

页数：8

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