Exoglucanase production by Aspergillus niger grown on wheat bran

被引:17
|
作者
Chandra, Muniramanna Gari Subhosh [1 ]
Reddy, Bontha Rajasekhar [1 ]
机构
[1] Sri Krishnadevaraya Univ, Dept Microbiol, Anantapur 515003, AP, India
关键词
Solid state fermentation; Exoglucanase; Aspergillus niger; Wheat bran; SOLID-STATE FERMENTATION; CELLULOLYTIC ENZYMES; CELLULASE;
D O I
10.1007/s13213-012-0538-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
beta-Exoglucanase production on the lignocellulosic material, wheat bran, by Aspergillus niger under solid state fermentation (SSF) on a laboratory scale was investigated. Different fermentation parameters, such as moisture content, initial pH, temperature, depth of the substrate, and inoculum size on exoglucanase production were optimized. Moisture content of 40 %, pH of 7.0, substrate depth of 1.0 cm, inoculum size of 2 x 10(6) spores/g of wheat bran, and temperature at 30 A degrees C were optimal for maximum production of exoglucanase. Maximum yields of exoglucanase with 28.60 FPU/g of wheat bran were obtained within 3 days of incubation under optimal conditions.
引用
收藏
页码:871 / 877
页数:7
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