Differential gene expression of human CD34+ hematopoietic stem and progenitor cells before and after culture

被引：11

作者：

Li, QL ^{[1
]}

Cai, HB ^{[1
]}

Liu, QW ^{[1
]}

Tan, WS ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 06期

关键词：

cDNA array; CD34(+) hematopoietic stem cells; cord blood; ex vivo expansion; SMART-PCR;

D O I：

10.1007/s10529-005-6064-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ex vivo expanded CD34(+) hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34(+) cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34(+) HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.

引用

页码：389 / 394

页数：6

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