Differential gene expression of human CD34+ hematopoietic stem and progenitor cells before and after culture

被引：11
|
作者：
Li, QL [1 ]
Cai, HB [1 ]
Liu, QW [1 ]
Tan, WS [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 06期
关键词：
cDNA array; CD34(+) hematopoietic stem cells; cord blood; ex vivo expansion; SMART-PCR;
D O I：
10.1007/s10529-005-6064-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Ex vivo expanded CD34(+) hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34(+) cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34(+) HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.
引用
收藏
页码：389 / 394
页数：6
相关论文
共 50 条
  • [1] Differential Gene Expression of Human CD34+ Hematopoietic Stem and Progenitor Cells Before and After Culture
    Qunliang Li
    Haibo Cai
    Qiwei Liu
    Wen-Song Tan
    [J]. Biotechnology Letters, 2006, 28 : 389 - 394
  • [2] The pattern of gene expression in human CD34+ stem/progenitor cells
    Zhou, GL
    Chen, JJ
    Lee, S
    Clark, T
    Rowley, JD
    Wang, SM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (24) : 13966 - 13971
  • [3] The transcriptome of human CD34+ hematopoietic stem-progenitor cells
    Kim, Yeong C.
    Wu, Qingfa
    Chen, Jun
    Xuan, Zhenyu
    Jung, Yong-Chul
    Zhang, Michael Q.
    Rowley, Janet D.
    Wang, San Ming
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (20) : 8278 - 8283
  • [4] Differential gene expression profiling of CD34+ CD133+ umbilical cord blood hematopoietic stem progenitor cells
    He, XH
    Gonzalez, V
    Tsang, A
    Thompson, J
    Tsang, TC
    Harris, DT
    [J]. STEM CELLS AND DEVELOPMENT, 2005, 14 (02) : 188 - 198
  • [5] Myelopoiesis specific gene expression profiling in human CD34+ hematopoietic stem cells
    Joshi, Madhuri
    Gangenahalli, Gurudutta
    [J]. GENE EXPRESSION PATTERNS, 2020, 37
  • [6] A comparative gene-expression analysis of CD34+ hematopoietic stem and progenitor cells grown in static and stirred culture systems
    Qunliang Li
    Qiwei Liu
    Haibo Cai
    Wen-Song Tan
    [J]. Cellular & Molecular Biology Letters, 2006, 11 : 475 - 487
  • [7] A comparative gene-expression analysis of CD34+ hematopoietic stem and progenitor cells grown in static and stirred culture systems
    Li, Qunliang
    Liu, Qiwei
    Cai, Haibo
    Tan, Wen-Song
    [J]. CELLULAR & MOLECULAR BIOLOGY LETTERS, 2006, 11 (04) : 475 - 487
  • [8] A comparative gene-expression analysis of CD34+ hematopoietic stem and progenitor cells grown in static and stirred culture systems
    Qunliang Li
    Qiwei Liu
    Haibo Cai
    Wen-Song Tan
    [J]. Cellular & Molecular Biology Letters, 2006, 11 (4) : 616 - 616
  • [9] A candidate cytokine gene specifically expressed in CD34+ human hematopoietic stem/progenitor cells.
    Liu, X
    Rapp, N
    Deans, R
    Cheng, LZ
    [J]. BLOOD, 1999, 94 (10) : 141B - 141B
  • [10] Differential gene expression underlying the functional distinctions of primary human CD34+ hematopoietic stem and progenitor cells from peripheral blood and bone marrow
    Steidl, U
    Kronenwett, R
    Haas, R
    [J]. HEMATOPOIETIC STEM CELLS 2002: GENETICS AND FUNCTION, 2003, 996 : 89 - 100
12345