The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1

被引：23

作者：

Hasebe, Ai ^{[1
]}

Nakamura, Yoko ^{[1
]}

Tashima, Hiroki ^{[1
]}

Takahashi, Kaneyoshi ^{[1
]}

Iijima, Masumi ^{[1
]}

Yoshimoto, Nobuo ^{[1
]}

Ting, Kang ^{[2
]}

Kuroda, Shun'ichi ^{[1
]}

Niimi, Tomoaki ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[2] Univ Calif Los Angeles, Dent & Craniofacial Res Inst, Los Angeles, CA 90095 USA

来源：

FEBS LETTERS | 2012年 / 586卷 / 16期

关键词：

NELL-1; Cell adhesion; Extracellular matrix; Osteoblast differentiation; EXTRACELLULAR MODULATORS; MATRICELLULAR PROTEINS; LEISHMANIA-TARENTOLAE; EXPRESSION; DIFFERENTIATION; THROMBOSPONDIN-1; CRANIOSYNOSTOSIS; MINERALIZATION; IDENTIFICATION; REGENERATION;

D O I：

10.1016/j.febslet.2012.06.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NELL1 is a secretory osteogenic protein containing several structural motifs that suggest that it functions as an extracellular matrix component. To determine the mechanisms underlying NELL1-induced osteoblast differentiation, we examined the cell-adhesive activity of NELL1 using a series of recombinant NELL1 proteins. We demonstrated that NELL1 promoted osteoblastic cell adhesion through at least three cell-binding domains located in the C-terminal region of NELL1. Adhesion of cells to NELL1 was strongly inhibited by function-blocking antibodies against integrin alpha 3 and beta 1 subunits, suggesting that osteoblastic cells adhered to NELL1 through integrin alpha 3 beta 1. Further, focal adhesion kinase activation is involved in NELL1 signaling. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2500 / 2506

页数：7

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