Detection of a single copy gene on a mitotic metaphase chromosome by fluorescence in situ hybridization (FISH) in the sawfly, Athalia rosae (Hymenoptera)

被引：10
|
作者：
Matsumoto, K
Yamamoto, DS
Sumitani, M
Lee, JM
Hatakeyama, M
Oishi, K [1 ]
机构：
[1] Kobe Univ, Fac Sci, Dept Biol, Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Grad Sch Sci & Technol, Div Biol, Nada Ku, Kobe, Hyogo 657, Japan
[3] Kobe Univ, Grad Sch Sci & Technol, Div Biosci, Nada Ku, Kobe, Hyogo 657, Japan
[4] Natl Inst Agrobiol Sci, Inst Insect & Anim Sci, Dept Dev Biol, Dev Mech Lab, Tsukuba, Ibaraki, Japan
来源：
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 2002年 / 49卷 / 01期
关键词：
FISH; rDNA; vitellogenin; cDNA; Athalia rosae; Hymenoptera;
D O I：
10.1002/arch.10005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mitotic metaphase chromosomes of Athalia rosae (Hymenoptera) haploid males were subjected to fluorescence in situ hybridization (FISH) analysis using an rDNA probe and two vitellogenin (Vg) cDNA probes (one representing the 5' half and the other the 3' half of the gene, each about 3 kb long, and together covering the entire coding region). The rDNA probe produced signals in four chromosomes, all in pericentromeric regions (haploid chromosome number = 8), and the Vg probes, either the combined probes or the 3' region alone, produced a twin signal in the middle of a chromosome arm of a single chromosome. Arch. Insect Biochem. Physiol. 49:34-40, 2002. (C) 2002 Wiley-Liss, Inc.
引用
收藏
页码：34 / 40
页数：7
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