Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)

被引:2
|
作者
Armstrong, Amelia J. [1 ]
Dudgeon, Christine L. [1 ]
Bustamante, Carlos [2 ]
Bennett, Michael B. [1 ]
Ovenden, Jennifer R. [1 ]
机构
[1] Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia
[2] Univ Arturo Prat, Fac Recursos Nat Renovables, Iquique, Chile
基金
澳大利亚研究理事会;
关键词
Population genetics; Conservation genetics; Microsatellite loci; Short tandem repeats;
D O I
10.1186/s13104-019-4270-8
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
ObjectiveLimited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of 17 novel microsatellite markers for the reef manta ray (Mobula alfredi).ResultsLoci were screened on 60 reef manta rays (M. alfredi) sampled from the east coast of Australia. The number of alleles per locus varied from 2 to 13 with observed heterozygosities ranging between 0.300 and 0.917. The development of these 17 additional markers increases the total number of microsatellite markers available for this species to 27.
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页数:5
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