A SIMULTANEOUS MULTI-PROBE DETECTION LABEL-FREE OPTICAL-RESOLUTION PHOTOACOUSTIC MICROSCOPY TECHNIQUE BASED ON MICROCAVITY TRANSDUCER

被引:3
|
作者
Wu, Yongbo [1 ]
Tang, Zhilie [1 ,2 ]
Chi, Yan [1 ]
Wu, Liru [1 ]
机构
[1] S China Normal Univ, Sch Phys & Telecom Engn, Guangzhou 510006, Guangdong, Peoples R China
[2] S China Normal Univ, Lab Quantum Informat Technol, IMOT, Guangzhou 510006, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Multi-probe; label-free; optical-resolution photoacoustic microscopy;
D O I
10.1142/S1793545813500272
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We demonstrate the feasibility of simultaneous multi-probe detection for an optical-resolution photoacoustic microscopy (OR-PAM) system. OR-PAM has elicited the attention of biomedical imaging researchers because of its optical absorption contrast and high spatial resolution with great imaging depth. OR-PAM allows label-free and noninvasive imaging by maximizing the optical absorption of endogenous biomolecules. However, given the inadequate absorption of some biomolecules, detection sensitivity at the same incident intensity requires improvement. In this study, a modulated continuous wave with power density less than 3 mW/cm(2) (1/4 of the ANSI safety limit) excited the weak photoacoustic (PA) signals of biological cells. A microcavity transducer is developed based on the bulk modulus of gas five orders of magnitude lower than that of solid; air pressure variation is inversely proportional to cavity volume at the same temperature increase. Considering that a PA wave expands in various directions, detecting PA signals from different positions and adding them together can increase detection sensitivity and signal-to-noise ratio. Therefore, we employ four detectors to acquire tiny PA signals simultaneously. Experimental results show that the developed OR-PAM system allows the label-free imaging of cells with weak optical absorption.
引用
收藏
页数:6
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