Purpose: To investigate prevalence and risk factors of nocturia and quality of life of nocturia subjects from Shanghai. Methods: Investigations by questionnaire were conducted in 3,646 people over 50 years old. The risk factors including the age, hypertension, diabetes and benign prostatic hyperplasia (BPH) etc were also evaluated. Results: 1,332 urinated more than twice each night (36.5%), and average age was 72.06. The increased frequency of night urination makes the hours of uninterrupted sleep (HUS) reduce significantly (P<0.001). Overactive bladder (OAB) was a risk factor of nocturia (P<0.001); OAB symptom scores were significantly different between nocturia subjects (urination twice, thrice and quartic) and others (P12 <= 0.001, P13 =0.002, P14<0.001). The frequent night urination deteriorated the quality of life and sleeping. Hypertension, diabetes, snoring and BPH are risk factors of nocturia. Conclusion: The increased night urination impacts the HUS, quality of life and sleep quality. The age, hypertension, diabetes, BPH, snoring and OAB are the risk factors of nocturia.