Investigation of molecular diffusion in hydrogel by electronic speckle pattern interferometry

被引:29
|
作者
Zhang, XM
Hirota, N
Narita, T
Gong, JP
Osada, Y [1 ]
Chen, KS
机构
[1] Hokkaido Univ, Div Biol Sci, Grad Sch Sci, Sapporo, Hokkaido 0600810, Japan
[2] Zhejiang Univ, Dept Informat & Elect Engn, Hangzhou 310027, Peoples R China
来源
JOURNAL OF PHYSICAL CHEMISTRY B | 1999年 / 103卷 / 29期
关键词
D O I
10.1021/jp983866o
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The technique of real-time electronic speckle pattern interferometry (ESPI) is proposed to study the diffusion process of surfactants and proteins in gels. The diffusion coefficient is simply and directly determined from the interferograms. The experiments were carried out on a series of surfactants as well as on a myoglobin diffusion in agarose gels. The agreement of the diffusion coefficient values obtained from ESPI with those obtained by electrical conductance: measurement or the reported data demonstrates the usefulness of the method. Moreover, spatial distribution of refractive index change in the gel can be determined by phase evaluation. Fick's law for diffusion is utilized to analyze the spatial distribution of the diffuser concentration curves in gel, which are compared with experimental data. We find that the agarose gel in surfactant solution shrinks slightly, which increases the density of the gel and leads to the additional refractive index change. The effect of gel shrinking to surfactant diffusion is discussed, and the distribution of density variation of the gel with time is evaluated.
引用
收藏
页码:6069 / 6074
页数:6
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