The AI-2/luxS Quorum Sensing System Affects the Growth Characteristics, Biofilm Formation, and Virulence of Haemophilus parasuis

被引:58
|
作者
Zhang, Bingzhou [1 ]
Ku, Xugang [1 ]
Zhang, Xiaoqian [1 ]
Zhang, Yan [2 ]
Chen, Guo [1 ]
Chen, Fangzhou [1 ]
Zeng, Wei [1 ]
Li, Jing [1 ]
Zhu, Ling [1 ]
He, Qigai [1 ]
机构
[1] Huazhong Agr Univ, State Key Lab Agr Microbiol, Div Anim Infect Dis, Coll Anim Sci & Vet Med, Wuhan, Hubei, Peoples R China
[2] Xinjiang Agr Univ, Coll Anim Sci & Vet Med, Urumqi, Peoples R China
来源
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2019年 / 9卷
关键词
quorum sensing; H; parasuis; luxS; biofilm formation; virulence; MANNHEIMIA-HAEMOLYTICA A1; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; SERUM-RESISTANCE; STREPTOCOCCUS-MUTANS; STRESS TOLERANCE; KEY ROLE; LUXS; SIGNAL; AUTOAGGLUTINATION; AUTOINDUCER-2;
D O I
10.3389/fcimb.2019.00062
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Haemophilus parasuis (H. parasuis) is a kind of opportunistic pathogen of the upper respiratory tract of piglets. Under certain circumstances, virulent strains can breach the mucosal barrier and enter the bloodstream, causing severe Glasser's disease. Many virulence factors are found to be related to the pathogenicity of H. parasuis strain, but the pathogenic mechanism remains unclear. LuxS/AI-2, as a kind of very important quorum sensing system, affects the growth characteristics, biofilm formation, antibiotic production, virulence, andmetabolismof different strains. In order to investigate the effect of luxS/AI-2 quorum sensing system on the virulence of H. parasuis, a deletion mutant strain (1 luxS) and complemented strain (C-luxS) were constructed and characterized. The results showed that the luxS gene participated in regulating and controlling stress resistance, biofilm formation and virulence. Compared with wild-type strain, 1 luxS strain decreased the production of AI-2 molecules and the tolerance toward oxidative stress and heat shock, and it reduced the abilities of autoagglutination, hemagglutination, and adherence, whereas it increased the abilities to form biofilm in vitro. In vivo experiments showed that 1 luxS strain attenuated its virulence about 10-folds and significantly decreased its tissue burden of bacteria inmice, compared with the wild-type strain. Taken together, the luxS/AI-2 quorum sensing system in H. parasuis not only plays an important role in growth and biofilm formation, but also affects the pathogenicity of H. parasuis
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页数:15
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