Development and characterization of eight polymorphic microsatellite markers for Daphnia atkinsoni (Crustacea: Ctenodaphnia)

被引：2
|
作者：
Ortells, R. [1 ]
Van Houdt, J. K. J. [2 ]
Geldof, S. [1 ]
De Meester, L. [1 ]
Mergeay, J. [1 ]
机构：
[1] Katholieke Univ Leuven, Lab Aquat Ecol & Evolutionary Biol, B-3000 Louvain, Belgium
[2] Royal Museum Cent Africa, Joint Expt Mol Unit, B-3080 Tervuren, Belgium
来源：
MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 01期
关键词：
Cladocera; Daphnia atkinsoni; Daphnia bolivari; ecological genetics; microsatellites; population structure; GENETIC-STRUCTURE; POPULATIONS;
D O I：
10.1111/j.1755-0998.2008.02375.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A microsatellite-enriched genomic library was developed for the water flea Daphnia atkinsoni Baird, 1859, a dominant species of intermittent wetlands in Europe. Eight polymorphic microsatellite markers were successfully optimized. Characterization of 77 individuals from Belgium and Spain showed moderate (in the former) to high (in the latter) levels of polymorphism with two to 11 alleles per locus and an observed heterozygosity ranging from 0 to 0.87. Some of these microsatellite markers were successfully amplified in three other Daphnia species (D. magna n = 4, D. similis n = 6; D. obtusa n = 6).
引用
收藏
页码：326 / 329
页数：4
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