Drying Enhances Signal Intensities for Global GC-MS Metabolomics

被引:14
|
作者
Liebeke, Manuel [1 ]
Puskas, Erik [1 ]
机构
[1] Max Planck Inst Marine Microbiol, Celsiusstr 1, D-28359 Bremen, Germany
来源
METABOLITES | 2019年 / 9卷 / 04期
关键词
metabolomics; sample preparation; gas chromatography; derivatization; silylation; CHROMATOGRAPHY-MASS SPECTROMETRY; DERIVATIZATION; METABOLITES; EXTRACTION; PLATFORM; TISSUE;
D O I
10.3390/metabo9040068
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report here that a straightforward change of the standard derivatization procedure for GC-MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware.
引用
收藏
页数:8
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