Drying Enhances Signal Intensities for Global GC-MS Metabolomics

被引：14
|
作者：
Liebeke, Manuel [1 ]
Puskas, Erik [1 ]
机构：
[1] Max Planck Inst Marine Microbiol, Celsiusstr 1, D-28359 Bremen, Germany
来源：
METABOLITES | 2019年 / 9卷 / 04期
关键词：
metabolomics; sample preparation; gas chromatography; derivatization; silylation; CHROMATOGRAPHY-MASS SPECTROMETRY; DERIVATIZATION; METABOLITES; EXTRACTION; PLATFORM; TISSUE;
D O I：
10.3390/metabo9040068
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report here that a straightforward change of the standard derivatization procedure for GC-MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware.
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页数：8
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