Biodiesel production using cross-linked Staphylococcus haemolyticus lipase immobilized on solid polymeric carriers

被引:30
|
作者
Kim, Sang Hyeon [1 ]
Kim, Soon-ja [1 ]
Park, Seongsoon [2 ]
Kim, Hyung Kwoun [1 ]
机构
[1] Catholic Univ Korea, Dept Biotechnol, Puchon 420743, Gyeonggi, South Korea
[2] Sungshin Womens Univ, Ctr NanoBio Appl Technol, Inst Basic Sci, Dept Chem, Seoul 136742, South Korea
关键词
Staphylococcus haemolyticus; Lipase; Biodiesel; Transesterification; Immobilization; CANDIDA-ANTARCTICA LIPASE; RHIZOPUS-ORYZAE LIPASE; BIOCHEMICAL-PROPERTIES; ENZYMATIC PRODUCTION; ORGANIC-SOLVENT; VEGETABLE-OIL; SYSTEM; ADSORPTION; BUTANOL; WATER;
D O I
10.1016/j.molcatb.2012.08.012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Various plant oils and animal fats can be used for the production of biodiesel, a renewable and eco-friendly alternative fuel. In this research, transesterification of olive oil and methanol was performed using Staphylococcus haemolyticus L62 lipase expressed in Escherichia coli cells. L62 lipase was relatively methanol-stable and could produce biodiesel by a one-step methanol-feeding process. This enzyme was immobilized on a poly (methacrylate-co-divinyl benzene) resin by two different methods: hydrophobic adsorption/entrapment (H-L62) and hydrophobic adsorption/entrapment plus covalent cross-linking (HC-L62). Both immobilized enzymes showed quite increased temperature and pH stabilities, whereas they had very similar optimal temperature and pH in comparison with the soluble free enzyme. Soluble L62, H-L62 and HC-L62 lipases could produce biodiesel efficiently from olive oil. Among them, HC-L62 lipase was the most robust enzyme on the basis of multiple reusability. HC-L62 produced biodiesel with various plant oils including palm, olive fomase oils as well as waste cooking oil, suggesting that it can be utilized as a biocatalyst in the biodiesel industry. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:10 / 16
页数:7
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