Recognition imaging with a DNA aptamer

被引：46

作者：

Lin, Liyun

Wang, Hongda

Liu, Yan

Yan, Hao

Lindsay, Stuart ^{[1
]}

机构：

[1] Arizona State Univ, Dept Phys, Tempe, AZ 85281 USA

[2] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85281 USA

[3] Arizona State Univ, Biodesign Inst, Tempe, AZ 85281 USA

来源：

BIOPHYSICAL JOURNAL | 2006年 / 90卷 / 11期

关键词：

D O I：

10.1529/biophysj.105.079111

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (similar to 90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

引用

页码：4236 / 4238

页数：3

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