Thiamin biosynthesis in eukaryotes:: Characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis

被引：44
|
作者：
Chatterjee, Abhishek [1 ]
Jurgenson, Christopher T. [1 ]
Schroeder, Frank C. [1 ]
Ealick, Steven E. [1 ]
Begley, Tadhg P. [1 ]
机构：
[1] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 22期
关键词：
D O I：
10.1021/ja061413o
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The biosynthesis of thiamin pyrophosphate in eukaryotes is different from the prokaryotic biosynthesis and is poorly understood to date. Only one thiazole biosynthetic gene has been identified (Thi4 in Saccharomyces cerevisiae). Here we report the identification and characterization of a Thi4-bound metabolite that consists of the ADP adduct of 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid. The unexpected structure of this compound yields the first insights into the mechanism of thiamin thiazole biosynthesis in eukaryotes. Copyright © 2006 American Chemical Society.
引用
收藏
页码：7158 / 7159
页数：2
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