Expression of Phosphorylated cAMP Response Element Binding Protein (p-CREB) in Bladder Afferent Pathways in VIP-/- Mice with Cyclophosphamide (CYP)-Induced Cystitis

被引：7
|
作者：
Jensen, Dorthe G. [2 ]
Studeny, Simon [1 ]
May, Victor [3 ]
Waschek, James [4 ]
Vizzard, Margaret A. [1 ,3 ]
机构：
[1] Univ Vermont, Coll Med, Dept Neurol, Burlington, VT 05405 USA
[2] Univ Copenhagen, Fac Pharmaceut Sci, Dept Pharmacol & Pharmacotherapy, Copenhagen, Denmark
[3] Univ Vermont, Coll Med, Dept Anat Neurobiol, Burlington, VT 05405 USA
[4] Univ Calif Los Angeles, Los Angeles, CA USA
来源：
JOURNAL OF MOLECULAR NEUROSCIENCE | 2008年 / 36卷 / 1-3期
关键词：
Inflammation; Urinary bladder; Afferent neurons; Growth factors; Cytokines;
D O I：
10.1007/s12031-008-9045-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The expression of phosphorylated cAMP response element binding protein ( p- CREB) in dorsal root ganglia ( DRG) with and without cyclophosphamide ( CYP)induced cystitis ( 150 mg/ kg, i. p; 48 h) was determined in VIP-/- and wild- type ( WT) mice. p- CREB immunoreactivity ( IR) was determined in bladder ( Fast blue) afferent cells. Nerve growth factor ( NGF) bladder content was determined by enzyme- linked immunosorbent assays. Basal expression of p- CREB- IR in DRG of VIP-/- mice was ( p= 0.01) greater in L1, L2, L5- S1 DRG compared to WT mice. CYP treatment in WT mice increased ( p= 0.05) p- CREB- IR in L1, L2, L5- S1 DRG. CYP treatment in VIP-/- mice ( p= 0.01) increased ( p= 0.01) p- CREB- IR in L6- S1 DRG compared to WT with CYP. In WT mice, bladder afferent cells ( 20 - 38%) in DRG expressed p- CREB- IR under basal conditions. With CYP, p- CREB- IR increased in bladder afferent cells ( 60 - 65%; L6- S1 DRG) in WT mice. In VIP-/mice, bladder afferent cells ( 12 - 58%) expressed p- CREBIR under basal conditions, and CYP increased p- CREB expression ( 78 - 84%) in L6- S1 DRG. Urinary bladder NGF expression in VIP-/- mice under basal conditions or after cystitis was significantly greater than WT. Detrusor smooth muscle thickness was significantly increased in VIP-/mice. Bladder NGF expression may contribute to differences in p- CREB expression.
引用
收藏
页码：299 / 309
页数：11
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