Variability of HIV-1 Genomes among Children and Adolescents from Sao Paulo, Brazil

被引:16
|
作者
Sanabani, Sabri Saeed [1 ,2 ]
Pessoa, Rodrigo [2 ]
Soares de Oliveira, Ana Carolina [2 ]
Martinez, Vanessa Pouza [2 ]
Giret, Maria Teresa Maidana [3 ]
de Menezes Succi, Regina Celia [4 ]
Carvalho, Karina [5 ]
Tomiyama, Claudia Satiko [5 ]
Nixon, Douglas F. [6 ]
Sabino, Ester Cerdeira [2 ]
Kallas, Esper Georges [5 ]
机构
[1] Univ Sao Paulo, Sch Med, Clin & Res Lab LIM 03, Sao Paulo, Brazil
[2] Univ Sao Paulo, Inst Trop Med, Virol Lab LIM HCFMUSP 52, Sao Paulo, Brazil
[3] Univ Florida, Miller Sch Med, Story Lab 2, Miami, FL USA
[4] Univ Fed Sao Paulo, Paulista Sch Med, Dept Pediat, Sao Paulo, Brazil
[5] Univ Sao Paulo, Sch Med, Div Clin Immunol & Allergy, Sao Paulo, Brazil
[6] Univ Calif San Francisco, Dept Med, Div Expt Med, San Francisco, CA USA
来源
PLOS ONE | 2013年 / 8卷 / 05期
基金
美国国家卫生研究院; 巴西圣保罗研究基金会;
关键词
IMMUNODEFICIENCY-VIRUS TYPE-1; CIRCULATING RECOMBINANT FORM; SUBTYPE-C; VERTICAL TRANSMISSION; MOLECULAR EPIDEMIOLOGY; GENOTYPIC RESISTANCE; DRUG-RESISTANCE; DUAL INFECTION; VIRAL LOAD; CLADE-B;
D O I
10.1371/journal.pone.0062552
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Genetic variability is a major feature of the human immunodeficiency virus type 1 (HIV-1) and considered the key factor to frustrating efforts to halt the virus epidemic. In this study, we aimed to investigate the genetic variability of HIV-1 strains among children and adolescents born from 1992 to 2009 in the state of Sao Paulo, Brazil. Methodology: Plasma and peripheral blood mononuclear cells (PBMC) were collected from 51 HIV-1-positive children and adolescents on ART followed between September 1992 and July 2009. After extraction, the genetic materials were used in a polymerase chain reaction (PCR) to amplify the viral near full length genomes (NFLGs) from 5 overlapped fragments. NFLGs and partial amplicons were directly sequenced and data were phylogenetically inferred. Results: Of the 51 samples studied, the NFLGs and partial fragments of HIV-1 from 42 PBMCs and 25 plasma were successfully subtyped. Results based on proviral DNA revealed that 22 (52.4%) patients were infected with subtype B, 16 (38.1%) were infected with BF1 mosaic variants and 4 (9.5%) were infected with sub-subtype F1. All the BF1 recombinants were unique and distinct from any previously identified unique or circulating recombinant forms in South America. Evidence of dual infections was detected in 3 patients coinfected with the same or distinct HIV-1 subtypes. Ten of the 31 (32.2%) and 12 of the 21 (57.1%) subjects with recovered proviral and plasma, respectively, protease sequences were infected with major mutants resistant to protease inhibitors. The V3 sequences of 14 patients with available sequences from PBMC/or plasma were predicted to be R5-tropic virus except for two patients who harbored an X4 strain. Conclusions: The high proportion of HIV-1 BF1 recombinant, coinfection rate and vertical transmission in Brazil merits urgent attention and effective measures to reduce the transmission of HIV among spouses and sex partners.
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页数:15
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