The largest subfamily of Pyraloidea of the Galapagos fauna is reviewed. Forty-four species are recorded and illustrated, including the male and female genitalia. Eleven species are described as new: Agathodes galapagensis Landry sp. n., Desmia mordor Landry & Solis sp. n., Diaphania galapagensis Landry & Solis sp. n., Lineodes vulcanalis Landry sp. n., L. corinnae Landry sp. n., Neoleucinodes galapagensis Landry sp. n., Samea coffea Landry sp. n., Sanwa inconspicuella Landry sp. n., Sisyracera jacquelinae Landry sp. n., Udea galapagensis Landry sp. n., Udea sideralis Landry sp. n. Asciodes quietalis (Walker, 1859), stat. n. and Samea castellalis Guenee, 1854, stat. n. are removed from synonymy. Samea contortilinealis Hampson, 1895 is synonymized with Sisyracera inabsconsalis (Moschler, 1890). Lectotypes are designated for Botys cambogialis Guenee, 1854, Phlyctaenia capsifera Meyrick, 1933, Phalaena costata Fabricius, 1775, Botys creonalis Walker, 1859, Samea disertalis Walker, 1866, Asciodes gordialis Guenee, 1854, Diasemia inabsconsalis Moschler, 1890, Samea jarbusalis Walker, 1859, Botys philealis Walker, 1859, and Scoparia quietalis Walker, 1859. The known distribution and biology of each species are summarized. Cryptobotys zoilusalis (Walker, 1859) and Salbia haemorrhoidalis Guenee, 1854 are recorded from the Galapagos for the first time. A host plant record is mentioned for Penestola bufalis (Guenee, 1854) for the first time. The binomen Patania silicalis (Guenee, 1854), comb. n., is mentioned for the first time.