Characterization of influenza A virus with nine segments: Effect gene segment on virus property

被引：1
|
作者：
Wu, Rui [1 ,2 ,3 ]
Zhang, Xuke [3 ]
Shao, Huabin [1 ,2 ]
Luo, Qingping [1 ]
Yang, Jun [1 ]
Xu, Diping [1 ,2 ]
机构：
[1] Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430064, Hubei, Peoples R China
[2] Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Hubei, Peoples R China
[3] Luoyang Pulike Bioengn Co Ltd, Natl Res Ctr Vet Med, Luoyang 471003, Henan, Peoples R China
来源：
RESEARCH IN VETERINARY SCIENCE | 2012年 / 93卷 / 02期
关键词：
Influenza virus; H9N2; Nine segments; Virus property; ION-CHANNEL ACTIVITY; RNA SEGMENTS; VIRAL-RNA; PACKAGING SIGNALS; NONCODING REGIONS; VIRIONS; PROTEIN; REPLICATION; GENOME; HEMAGGLUTININ;
D O I：
10.1016/j.rvsc.2011.12.020
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The influenza A virus genome consists of eight segments of negative-strand RNA. In previous study, we generated a recombinant influenza virus with nine segments by reverse genetics. In present study, we evaluated characteristics of the recombinant influenza virus. The recombinant virus exhibited similar property to wild-type virus on virion morphology, virion composition, plaque phenotype and other aspects. Whereas, the recombinant virus propagated to lower titers than did wild-type virus in cells and mice, and there was decreased protein level and vRNA incorporation in the recombinant virions compared to wild-type H9N2 virions. Our results indicated that influenza A virus with eight segments exhibits more advantages than the virus with nine segments. (C) 2012 Published by Elsevier Ltd.
引用
收藏
页码：1076 / 1080
页数：5
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