Characterization of a pro-angiogenic, novel peptide from Russell's viper (Daboia russelii russelii) venom

被引：12
|
作者：
Mukherjee, Ashis K. [1 ,4 ]
Chatterjee, Suvro [2 ]
Majumder, Syamantak [2 ]
Saikia, Debashree [1 ]
Thakur, Rupamoni [1 ]
Chatterjee, Anupam [3 ]
机构：
[1] Tezpur Univ, Sch Sci, Dept Mol Biol & Biotechnol, Microbial Biotechnol & Prot Res Lab, Tezpur 784028, India
[2] Anna Univ, AU KBC Res Ctr, Madras 600044, Tamil Nadu, India
[3] North Eastern Hill Univ, Dept Biotechnol & Bioinformat, Mol Genet Lab, Shillong 793022, Meghalaya, India
[4] Univ No Colorado, Sch Biol Sci, Greeley, CO 80639 USA
来源：
TOXICON | 2014年 / 77卷
关键词：
Angiogenesis; Chromosomal aberration; Cytotoxicity; Russell's viper; Wound healing; IN-VITRO; MEDICINE; PROTEIN;
D O I：
10.1016/j.toxicon.2013.10.024
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Present report shows for the first time on the induction of in vitro angiogenesis by a 3.9 kDa novel peptide (RVVAP) purified from Russell's viper venom. Secondary structure of RVVAP is made up of 36.8% alpha-helix, 33.3% beta pleated sheets and 29.9% turns. Optimum angiogenesis and significant elevation in endothelial migration were observed at 50 ng/ml of RVVAP treatment; above this concentration, progressive decrease in wound healing was noted. RVVAP (1.0 mu g/ml) was non-cytotoxic to U87-MG, HeLa and HT-29 cells; however, increasing the RVVAP concentration above 500 ng/ml resulted in induction of chromosomal aberrations and delay in cell cycle kinetics of Chinese hamster ovary cells. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：26 / 31
页数：6
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