S-perindopril assay using a potentiometric, enantioselective membrane electrode

被引：0
|
作者：
Stefan, RI
Van Staden, JF [1 ]
Aboul-Enein, HY
机构：
[1] Univ Pretoria, Dept Chem, ZA-0002 Pretoria, South Africa
[2] King Faisal Specialist Hosp & Res Ctr, Biol & Med Res Dept MBC03, Bioanalyt & Drug Dev Lab, Riyadh 11211, Saudi Arabia
来源：
CHIRALITY | 1999年 / 11卷 / 08期
关键词：
S-perindopril; enantioselective analysis; potentiometric; enantioselective membrane electrode;
D O I：
10.1002/(SICI)1520-636X(1999)11:8<631::AID-CHIR4>3.0.CO;2-K
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A potentiometric, enantioselective membrane electrode based on graphite paste (graphite powder and paraffin oil) has been constructed. The graphite paste is impregnated with a 10(-3) mol/L 2-hydroxy-3-trimethylammoniopropyl-beta-cyclodextrin (as chloride salt) solution. The potentiometric, enantioselective membrane electrode can be used reliably for enantiopurity tests of S-perindopril using a chronopotentiometric (zero current) technique, in the 10(-5)-10(-2) mol/L concentration range (detection limit 5 x 10(-6) mol/L), with an average recovery of 99.58% (RSD = 0.33%). The enantioselectivity was determined over R-perindopril and D-proline. The response characteristics of the enantioselective, potentiometric membrane electrode were also determined for R-perindopril. It was shown that L-proline is the main interfering compound. The surface of the electrode can be regenerated simply by polishing, obtaining a fresh surface ready to be used in a new assay. (C) 1999 Wiley-Liss, Inc.
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页码：631 / 634
页数：4
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