At two sites of stationary experiments at Lipa (cambisol) and at Svitavy (Iuvisol) pH of soils, mineral and mineralizable N content in soils in spring and autumn period, yield of crops and N uptake of crops were evaluated. Different rates of fertilizers were applied at Lipa from 1974 and at Svitavy from 1980. The article evaluated three treatments (treatment 1 - unfertilized, treatment 2 - manure + NPK, treatment 3 - manure + NPK + liming). The following crops were cultivated during the period under study: 1991 - red clover, 1992 - winter wheat, 1993 - potatoes, 1994 - winter wheat, 1995 - spring barley. The results of investigations for the period 1991 to 1995 show that the yields were significantly affected by red clover, followed by manuring and fertilizing. Treatment 2 (manure + NPK) gave increase of the yield at Lipa by 62.3% compared with untreated treatment and treatment 3 (limed, in addition) by 84.1% and at Svitavy by 35.7% and 57.9%. Similar influence, but at lower level, was found in N uptake by crops. Fertilization affected the yield more than N uptake by plants. Different fertilization had an impact on pH of soils and mineral and mineralizable N in soils, hence also crop yields and N uptake by crops. Liming maintained favourable level of soil pH and increased the yields over five yields at Lips by 4.5 t and by 5.6 t of cereal units per 1 ha at Svitavy.