PROCESS OPTIMIZATION FOR AMYLOGLUCOSIDASE BY A MUTANT STRAIN OF ASPERGILLUS NIGER IN STIRRED FERMENTER

被引：0
|
作者：
Malik, Shazia [1 ,3 ]
Iftikhar, Tehreema [2 ]
ul Haq, Ikram [1 ]
Khattak, Manzoor Iqbal
机构：
[1] Govt Coll Univ, Inst Ind Biotechnol, Lahore, Pakistan
[2] Govt Coll Univ, Dept Bot, Faisalabad, Pakistan
[3] Queen Mary Coll, Dept Bot, Lahore, Pakistan
来源：
PAKISTAN JOURNAL OF BOTANY | 2013年 / 45卷 / 02期
关键词：
SOLID-STATE FERMENTATION; GLUCOAMYLASE PRODUCTION; SACCHARIFICATION; BIOSYNTHESIS; PROTEIN; AMYLASE; PH;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The present study was designed to optimize the process parameters for the production of amyloglucosidase bya mutant strain of Aspergillus niger in stirred fermenter by. For this purpose, various cultural conditions like rate of fermentation, process pH, rate of agitation and size of inoculum was investigated. The maximum production (25.15 U/mL/min) of amyloglucosidase was achieved at the agitation speed of 200rpm. The production of amyloglucosidase was found to be maximum (25.08 U/mL/min) at pH 5 of the medium. The optimum productivity (25.15 U/mL/min) of the enzyme was achieved with 4% inoculum after 48 h of incubation. The process temperature was optimized at 30 degrees C throughout the study.
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页码：663 / 666
页数：4
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