Characterization of a New Series of Fluorescent Probes for Imaging Membrane Order

被引:53
|
作者
Kwiatek, Joanna M. [1 ,2 ]
Owen, Dylan M. [1 ,2 ]
Abu-Siniyeh, Ahmed [1 ,2 ]
Yan, Ping [3 ]
Loew, Leslie M. [3 ]
Gaus, Katharina [1 ,2 ]
机构
[1] Univ New S Wales, Ctr Vasc Res, Sydney, NSW, Australia
[2] Univ New S Wales, Australian Ctr Nanomed, Sydney, NSW, Australia
[3] Univ Connecticut, Ctr Hlth, Ctr Cell Anal & Modelling, Farmington, CT USA
来源
PLOS ONE | 2013年 / 8卷 / 02期
基金
澳大利亚研究理事会; 美国国家卫生研究院;
关键词
LIPID RAFTS; GENERALIZED POLARIZATION; PLASMA-MEMBRANE; LAURDAN FLUORESCENCE; CELL-MEMBRANES; LIVING CELLS; DOMAINS; MICROSCOPY; DI-4-ANEPPDHQ; MODEL;
D O I
10.1371/journal.pone.0052960
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Visualization and quantification of lipid order is an important tool in membrane biophysics and cell biology, but the availability of environmentally sensitive fluorescent membrane probes is limited. Here, we present the characterization of the novel fluorescent dyes PY3304, PY3174 and PY3184, whose fluorescence properties are sensitive to membrane lipid order. In artificial bilayers, the fluorescence emission spectra are red-shifted between the liquid-ordered and liquid-disordered phases. Using ratiometric imaging we demonstrate that the degree of membrane order can be quantitatively determined in artificial liposomes as well as live cells and intact, live zebrafish embryos. Finally, we show that the fluorescence lifetime of the dyes is also dependent on bilayer order. These probes expand the current palate of lipid excitation/emission wavelengths and possibly new opportunities in membrane biology.
引用
收藏
页数:7
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