Analysis of ATP and AMP binding to a DNA aptamer and its imidazole-tethered derivatives by surface plasmon resonance

被引:9
|
作者
Zhao, Jing [1 ]
Katsube, Satoshi [2 ]
Yamamoto, Junpei [2 ]
Yamasaki, Kazuhiko [1 ]
Miyagishi, Makoto [1 ]
Iwai, Shigenori [2 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Ibaraki 3058566, Japan
[2] Osaka Univ, Grad Sch Engn Sci, Div Chem, Toyonaka, Osaka 5608531, Japan
关键词
ULTRASENSITIVE DETECTION; IN-VITRO; MOLECULES; ADENOSINE; SELECTION; LIGANDS; ENZYME; STABILIZATION; INTERLEUKIN-6; APTASENSOR;
D O I
10.1039/c5an01347j
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Imidazole was tethered to the C5 position of thymine in an ATP-binding DNA aptamer with two types of linkers, and the affinities of each aptamer for ATP and AMP were determined by surface plasmon resonance measurements. The imidazole-tethered aptamers exhibited higher affinity for ATP, almost independently of the linker structure or the modification site.
引用
收藏
页码:5881 / 5884
页数:4
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