Strong and weak zinc binding sites in human zinc-α2-glycoprotein

被引：23

作者：

Kumar, Aditya Arun ^{[1
]}

Hati, Debolina ^{[1
]}

Thaker, Thana'a Mohajer ^{[1
]}

Miah, Layeque ^{[1
]}

Cunningham, Phil ^{[1
]}

Domene, Carmen ^{[2
,4
]}

Bui, Tam T. T. ^{[5
]}

Drake, Alex F. ^{[5
]}

McDermott, Lindsay C. ^{[3
]}

机构：

[1] Kings Coll London, Dept Biochem, London SE1 9NH, England

[2] Kings Coll London, Dept Chem, London SE1 9NH, England

[3] Kings Coll London, Diabet & Nutr Sci Div, London SE1 9NH, England

[4] Univ Oxford, Chem Res Lab, Oxford OX1 3TA, England

[5] Kings Coll London, Biomol Spect Ctr, Opt & Chiropt Spect Facil, London SE1 1UL, England

来源：

FEBS LETTERS | 2013年 / 587卷 / 24期

关键词：

Zinc alpha 2 glycoprotein; Adipokine; ICP-MS; Spectroscopy; Molecular modeling; LIGAND-BINDING; BOUND ZINC; ZN-ALPHA(2)-GLYCOPROTEIN; ZAG;

D O I：

10.1016/j.febslet.2013.10.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc-alpha 2-glycoprotein (ZAG) is an adipokine with an MHC class I-like protein fold. Even though zinc causes ZAG to precipitate from plasma during protein purification, no zinc binding has been identified to date. Using mass spectrometry, we demonstrated that ZAG contains one strongly bound zinc ion, predicted to lie close to the alpha 1 and alpha 2 helical groove. UV, CD and fluorescence spectroscopies detected weak zinc binding to holo-ZAG, which can bind up to 15 zinc ions. Zinc binding to 11-(dansylamino) undecanoic acid was enhanced by holo-ZAG. Zinc binding may be important for ZAG binding to fatty acids and the beta-adrenergic receptor. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3949 / 3954

页数：6

共 50 条

[21] Studies on the anti-obesity activity of zinc-α2-glycoprotein in the rat
S T Russell
M J Tisdale
International Journal of Obesity, 2011, 35 : 658 - 665
[22] Studies on the antiobesity effect of zinc-α2-glycoprotein in the ob/ob mouse
S T Russell
M J Tisdale
International Journal of Obesity, 2011, 35 : 345 - 354
[23] Studies on the anti-obesity activity of zinc-α2-glycoprotein in the rat
Russell, S. T.
Tisdale, M. J.
INTERNATIONAL JOURNAL OF OBESITY, 2011, 35 (05) : 658 - 665
[24] Is zinc-α2-glycoprotein a cardiovascular protective factor for patients undergoing hemodialysis?
Leal, Viviane O.
Lobo, Julie C.
Stockier-Pinto, Milena B.
Farage, Najla E.
Abdalla, Dulcineia S. P.
Leite Junior, Maurilo
Mafra, Denise
CLINICA CHIMICA ACTA, 2012, 413 (5-6) : 616 - 619
[25] Serum levels of the adipokine zinc-α2-glycoprotein are increased in chronic hemodialysis
Philipp, Anne
Kralisch, Susan
Bachmann, Anette
Lossner, Ulrike
Kratzsch, Juergen
Blueher, Matthias
Stumvoll, Michael
Fasshauer, Mathias
METABOLISM-CLINICAL AND EXPERIMENTAL, 2011, 60 (05): : 669 - 672
[26] Studies on the antiobesity effect of zinc-α2-glycoprotein in the ob/ob mouse
Russell, S. T.
Tisdale, M. J.
INTERNATIONAL JOURNAL OF OBESITY, 2011, 35 (03) : 345 - 354
[27] Role of β-Adrenergic Receptors in the Oral Activity of Zinc-α2-Glycoprotein (ZAG)
Russell, Steven T.
Tisdale, Michael J.
ENDOCRINOLOGY, 2012, 153 (10) : 4696 - 4704
[28] Postnatal expression of zinc-α2-glycoprotein in rat white and brown adipose tissue
Tzanavari, Theodora
Bing, Chen
Trayhurn, Paul
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2007, 279 (1-2) : 26 - 33
[29] Circulating Zinc-α2-glycoprotein levels and Insulin Resistance in Polycystic Ovary Syndrome
Yerui Lai
Jinhua Chen
Ling Li
Jingxia Yin
Junying He
Mengliu Yang
Yanjun Jia
Dongfang Liu
Hua Liu
Yong Liao
Gangyi Yang
Scientific Reports, 6
[30] Thyroid Hormone Upregulates Zinc-α2-glycoprotein Production in the Liver but Not in Adipose Tissue
Simo, Rafael
Hernandez, Cristina
Saez-Lopez, Cristina
Soldevila, Berta
Puig-Domingo, Manel
Selva, David M.
PLOS ONE, 2014, 9 (01):

← 1 2 3 4 5 →