Focal adhesion kinase (FAK) expression and phosphorylation in sea urchin embryos

被引：11
|
作者：
García, MG [1 ]
Toney, SJ [1 ]
Hille, MB [1 ]
机构：
[1] Univ Washington, Dept Biol, Seattle, WA 98195 USA
来源：
GENE EXPRESSION PATTERNS | 2004年 / 4卷 / 02期
关键词：
focal adhesion kinase; sea urchin; epithelium formation; epithelial; blastulae; embryogenesis; FAK-relatcd non-kinase; Pyk2;
D O I：
10.1016/j.modgep.2003.08.005
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have cloned three cDNA isoforms of focal adhesion kinase (FAK) from the sea urchin, Lytechinus variegatus. The sea urchin FAK is more closely related to FAK from other deuterostomes than from invertebrate protostomes or to cell adhesion kinase beta (CAKbeta/Pyk2/FAK2). FAK is expressed in all cells of sea urchin embryos by the 120-cell stage and strongly in blastulae. Phospho-FAK concentrates on basal surfaces of epithelial cells in early blastulae and Occurs in syncytial cables of primary mesenchyme cells (PMC). Inhibition of FAK by constructs of FAK-related non-kinase delays blastocoel expansion and early PMC ingression. These results suggest that FAK has roles in cell adhesion and in the shape and integrity of the epithelial cells in sea Urchin embryos. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：223 / 234
页数：12
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