Investigation of metallodrug-protein interactions by size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry (ICP-MS)

被引：93
|
作者：
Szpunar, J
Makarov, A
Pieper, T
Keppler, BK
Lobinski, R
机构：
[1] CNRS, F-64000 Pau, France
[2] Univ Vienna, Inst Inorgan Chem, A-1090 Vienna, Austria
来源：
ANALYTICA CHIMICA ACTA | 1999年 / 387卷 / 02期
关键词：
platinum; ruthenium; antitumor drugs; speciation; SEC; ICP-MS; proteins;
D O I：
10.1016/S0003-2670(99)00074-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The coupling of size-exclusion HPLC with ICP-MS was developed for the studies of the kinetics of metallodrug binding to human serum proteins. Two platinum- and three ruthenium-based drugs were investigated. Various SEC columns (of different lengths and with different packings) were compared for the separation of the protein-bound and unbound fractions of a metallodrug prior to on-line detection of the metal (Ru or Pt). The approach developed offers considerable advantages over the methods based on ultrafiltration followed by the off-line metal determination in terms of speed, simplicity, precision and selectivity regarding the molecular weight of the complexes involved. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：135 / 144
页数：10
相关论文
共 50 条
  • [1] Direct quantification of metal-organic interactions by size-exclusion chromatography (SEC) and inductively coupled plasma mass spectrometry (ICP-MS)
    Piatina, TB
    Hering, JG
    [J]. JOURNAL OF ENVIRONMENTAL QUALITY, 2000, 29 (06) : 1839 - 1845
  • [2] Determination of interactions between antibody biotherapeutics and copper by size exclusion chromatography (SEC) coupled with inductively coupled plasma mass spectrometry (ICP/MS)
    Luo, Yanxin
    Ronk, Michael
    Joubert, Marisa K.
    Semin, David
    Nashed-Samuel, Yasser
    [J]. ANALYTICA CHIMICA ACTA, 2019, 1079 : 252 - 259
  • [3] Neptunium determination by inductively coupled plasma mass spectrometry (ICP-MS)
    D. E. Vance
    V. F. Belt
    T. J. Oatts
    D. K. Mann
    [J]. Journal of Radioanalytical and Nuclear Chemistry, 1998, 234 : 143 - 147
  • [4] INDUCTIVELY-COUPLED PLASMA MASS-SPECTROMETRY (ICP-MS)
    DOUGLAS, DJ
    HOUK, RS
    [J]. PROGRESS IN ANALYTICAL ATOMIC SPECTROSCOPY, 1985, 8 (01): : 1 - 18
  • [5] Determination of plutonium by inductively coupled plasma mass spectrometry (ICP-MS)
    Tonouchi, S
    Habuki, H
    Katoh, K
    Yamazaki, K
    Hashimoto, T
    [J]. JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 2002, 252 (02) : 367 - 371
  • [6] Determination of plutonium by inductively coupled plasma mass spectrometry (ICP-MS)
    S. Tonouchi
    H. Habuki
    K. Katoh
    K. Yamazaki
    T. Hashimoto
    [J]. Journal of Radioanalytical and Nuclear Chemistry, 2002, 252 : 367 - 371
  • [7] Speciation of platinum metabolites in plants by size-exclusion chromatography and inductively coupled plasma mass spectrometry
    Klueppel, D
    Jakubowski, N
    Messerschmidt, J
    Stuewer, D
    Klockow, D
    [J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 1998, 13 (04) : 255 - 262
  • [8] Neptunium determination by inductively coupled plasma mass spectrometry (ICP-MS)
    Vance, DE
    Belt, VF
    Oatts, TJ
    Mann, DK
    [J]. JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 1998, 234 (1-2) : 143 - 146
  • [9] Identification of organically bound iodine in soil humic substances by size exclusion chromatography/inductively coupled plasma mass spectrometry (SEC/ICP-MS)
    Yamada, H
    Hisamori, I
    Yonebayashi, K
    [J]. SOIL SCIENCE AND PLANT NUTRITION, 2002, 48 (03) : 379 - 385
  • [10] AN IMPROVED INTERFACE FOR INDUCTIVELY COUPLED PLASMA-MASS SPECTROMETRY (ICP-MS)
    DOUGLAS, DJ
    FRENCH, JB
    [J]. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 1986, 41 (03) : 197 - 204
12345