Passive protection of shrimp against white spot syndrome virus (WSSV) using specific antibody from egg yolk of chickens immunized with inactivated virus or a WSSV-DNA vaccine

被引：68
|
作者：
Lu, Yanan [1 ]
Liu, Junjun [1 ]
Jin, Liji [1 ,2 ]
Li, Xiaoyu [1 ]
Zhen, YuHong [1 ]
Xue, Hongyu [1 ]
You, Jiansong [1 ]
Xu, Yongping [1 ,2 ,3 ]
机构：
[1] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116024, Liaoning, Peoples R China
[2] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Liaoning, Peoples R China
[3] Ctr Food Safety Anim Origin, Minist Educ, Dalian, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2008年 / 25卷 / 05期
基金：
中国国家自然科学基金;
关键词：
White spot syndrome virus; DNA vaccine; Linker; CpG ODNs; Inactivated vaccine; IgY;
D O I：
10.1016/j.fsi.2008.08.010
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
White spot syndrome virus (WSSV) causes high mortality and large economic losses in cultured shrimp. The VP28. VP19 and VP15 genes encode viral structural proteins of WSSV. In this study, hens were immunized with recombinant plasmid (pCI-VP28/VP19/VP15) with linkers or with inactivated WSSV, which used CpG oligodeoxynucleotides (CpG ODNs) and Freund's adjuvant as adjuvant, respectively. Egg yolk immunoglobulin (IgY) from hens immunized with inactivated vaccine and DNA vaccine was obtained, purified and used for protection of Metapenaeus ensis shrimp against WSSV. The data showed that the antibody response of the hens immunized with the DNA vaccine was improved by CpG ODNs as adjuvant, but was still inferior to inactivated WSSV in both sera and egg yolks. Using specific IgY from hens immunized with inactivated WSSV and DNA vaccine to neutralize WSSV, the challenged shrimp showed 73.3% and 33.3% survival, respectively. Thus, the results suggest that passive immunization strategy with IgY will be a valuable method against WSSV infection in shrimp. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：604 / 610
页数：7
相关论文
共 50 条
  • [31] The antiviral vp28-siRNA expressed in bacteria protects shrimp against white spot syndrome virus (WSSV)
    Zhu, Fei
    Zhang, Xiaobo
    AQUACULTURE, 2011, 319 (3-4) : 311 - 314
  • [32] Yeast Surface Display of Two Proteins Previously Shown to Be Protective Against White Spot Syndrome Virus (WSSV) in Shrimp
    Ananphongmanee, Vorawit
    Srisala, Jiraporn
    Sritunyalucksana, Kallaya
    Boonchird, Chuenchit
    PLOS ONE, 2015, 10 (06):
  • [33] Transmission of white spot syndrome virus (WSSV) from Dendronereis spp. (Peters) (Nereididae) to penaeid shrimp
    Haryadi, D.
    Verreth, J. A. J.
    Verdegem, M. C. J.
    Vlak, J. M.
    JOURNAL OF FISH DISEASES, 2015, 38 (05) : 419 - 428
  • [34] Protection of Shrimp against White Spot Syndrome Virus (WSSV) with β-1,3-d-glucan-encapsulated vp28-siRNA Particles
    Fei Zhu
    Xiaobo Zhang
    Marine Biotechnology, 2012, 14 : 63 - 68
  • [35] Protection of Shrimp against White Spot Syndrome Virus (WSSV) with β-1,3-d-glucan-encapsulated vp28-siRNA Particles
    Zhu, Fei
    Zhang, Xiaobo
    MARINE BIOTECHNOLOGY, 2012, 14 (01) : 63 - 68
  • [36] Detection of white spot syndrome virus (WSSV) of shrimp by means of monoclonal antibodies (MAbs) specific to an envelope protein (28 kDa)
    Liu, W
    Wang, YT
    Tian, DS
    Yin, ZC
    Kwang, J
    DISEASES OF AQUATIC ORGANISMS, 2002, 49 (01) : 11 - 18
  • [37] Induction of Immunity and Resistance to White Spot Syndrome Virus (WSSV) in Shrimp Penaeus monodon (Fabricius) by Synthetic Oligodeoxynucleotide and Bacterial DNA
    Amar, Edgar C.
    Faisan, Joseph P., Jr.
    PHILIPPINE AGRICULTURAL SCIENTIST, 2012, 95 (03): : 267 - 277
  • [38] White spot syndrome virus (WSSV) detected by PCR in rotifers and rotifer resting eggs from shrimp pond sediments
    Yan, DC
    Dong, SL
    Huang, J
    Yu, XM
    Feng, MY
    Liu, XY
    DISEASES OF AQUATIC ORGANISMS, 2004, 59 (01) : 69 - 73
  • [39] Transmission of white spot syndrome virus (WSSV) to Litopenaeus vannamei from infected cephalothorax, abdomen, or whole shrimp cadaver
    Soto, MA
    Shervette, VR
    Lotz, JM
    DISEASES OF AQUATIC ORGANISMS, 2001, 45 (02) : 81 - 87
  • [40] DNA fragmentation, an indicator of apoptosis, in cultured black tiger shrimp Penaeus monodon infected with white spot syndrome virus (WSSV)
    Sahtout, AH
    Hassan, MD
    Shariff, M
    DISEASES OF AQUATIC ORGANISMS, 2001, 44 (02) : 155 - 159
12345