DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi

被引：345
|
作者：
Cubero, OF [1 ]
Crespo, A
Fatehi, J
Bridge, PD
机构：
[1] Univ Complutense, Dept Biol Vegetal 2, E-28040 Madrid, Spain
[2] CABI Biosci UK Ctr Egham, Egham TW20 9TY, Surrey, England
来源：
PLANT SYSTEMATICS AND EVOLUTION | 1999年 / 216卷 / 3-4期
关键词：
herbarium; DNA extraction; filamentous fungi; lichenized fungi;
D O I：
10.1007/BF01084401
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
This paper presents a DNA extraction method suitable for fresh, herbarium-stored, lichenized and other fungal specimens. The method is fast and reliable, comparatively inexpensive and is suitable for obtaining PCR amplification quality DNA from large numbers of samples in a short time. The method has been tested with over 300 samples of Ascochyta, Phyllosticta, Ramalina, Parmelia and Physconia. Amplifiable fungal DNA was extracted from pure cultures, leaf lesions, whole thalli and dissected "only-fungal" sections of lichenized fungi. In addition, the method has proved suitable for use with herbarium specimens of both lichenized and non-lichenized fungi, stored as dried pure cultures or dried infected plant material.
引用
收藏
页码：243 / 249
页数：7
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