Gold nanoparticle cytochrome c complexes: The effect of nanoparticle ligand charge on protein structure

被引:190
|
作者
Aubin-Tam, ME
Hamad-Schifferli, K
机构
[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA
[2] MIT, Biol Engn Div, Cambridge, MA 02139 USA
关键词
D O I
10.1021/la052102e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report the effect of nanoparticle ligand charge on the structure of a covalently, site-specifically linked protein. An nanoparticles with positive, negative, and neutral ligands were appended to a specific cysteine, C102, of Saccharomyces cerevisiae cytochrome c. Conjugates were purified by HPLC or gel electrophoresis. Circular dichroism spectroscopy shows that changing the nanoparticle ligand dramatically influences the attached cytochrome c structure. The protein retains its structure with neutral ligands but denatures in the presence of charged species. This is rationalized by the electrostatic interaction of amino acids in the local vicinity of C102 with the endgroups of the ligand.
引用
收藏
页码:12080 / 12084
页数:5
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