Species-specific PCR primers for detection of Xanthomonas vesicatoria

被引：8

作者：

Beran, Pavel ^{[1
]}

Mraz, Ivan ^{[2
]}

机构：

[1] Univ S Bohemia, Ctr Biotechnol, Fac Agr, Ceske Budejovice 37005, Czech Republic

[2] Acad Sci Czech Republic, Inst Plant Mol Biol, Ctr Biol, Dept Plant Virol, CR-37005 Ceske Budejovice, Czech Republic

来源：

CROP PROTECTION | 2013年 / 43卷

关键词：

Bacterial spot; Tomato; Pepper; atpD; Xanthomonads; CAMPESTRIS PV. VESICATORIA; BACTERIAL SPOT; TOMATO; PEPPER; RECLASSIFICATION; SEQUENCES; STRAINS;

D O I：

10.1016/j.cropro.2012.08.008

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

This study presents the development of a PCR method for specific detection of Xanthomonas vesicatoria, causal agent of bacterial spot of tomato and pepper. Primer pair XV1F and XV1R was designed based on partial DNA sequence of the catalytic subunit of the ATP synthase gene atpD and tested using a collection of various bacterial pathogens mainly for tomato and pepper. After optimization of the PCR conditions, the 365 bp long fragment was only produced in X vesicatoria samples. Therefore, the primers and protocol allow rapid and species-specific detection of X. vesicatoria. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：213 / 215

页数：3

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