Immobilization of Burkholderia Cepacia Lipase on Functionalized Ionic Liquids Modified Mesoporous Silica SBA-15

被引：6

作者：

Tang Susu ^{[1
]}

Hu Yi ^{[1
]}

Yu Dinghua ^{[1
]}

Zou Bin ^{[1
]}

Jiang Ling ^{[1
]}

机构：

[1] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China

来源：

CHINESE JOURNAL OF CATALYSIS | 2012年 / 33卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Burkholderia cepacia lipase; immobilization; mesoporous silica; ionic liquid; modification; ADSORPTION; CATALYSTS; ENZYME;

D O I：

10.3724/SP.J.1088.2012.20457

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Mesoporous silica SBA-15 was modified by imidazole-based ionic liquids with alkyl group. The modified support samples were characterized by nitrogen adsorption-desorption, small-angle X-ray diffraction, Fourier transform infrared spectroscopy, elemental analysis, and scanning electron microscopy. The samples were used to immobilize Burkholderia cepacia lipase (BCL) and the influence of alkyl chain length of ionic liquid on enzymatic properties was investigated by the hydrolysis reaction of triacetin. The results revealed that functionalized ionic liquid modification did not destroy the structure of SBA-15. Compared with the immobilized lipase on parent SBA-15, the immobilized lipases on modified support samples were less sensitive to temperature and low pH and showed higher specific activity and stability. There-into, BCL immobilized on methyl-functionalized ionic liquid modified SBA-15 had the highest specific activity, which improved 2.4 folds compared with BCL immobilized on SBA-15: BCL immobilized on octyl-functionalized ionic liquid modified SBA-15 had the best thermal stability, reusability, storage stability, and stability in organic solvent.

引用

页码：1565 / 1571

页数：7

共 26 条

[1] Biocatalytic esterification of citronellol with lauric acid by immobilized lipase on aminopropyl-grafted mesoporous SBA-15
Abdullah, A. Z.
Sulaiman, N. S.
Kamaruddin, A. H.
[J]. BIOCHEMICAL ENGINEERING JOURNAL, 2009, 44 (2-3) : 263 - 270
[2] Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres
Bai, YX
Li, YF
Yang, Y
Yi, LX
[J]. PROCESS BIOCHEMISTRY, 2006, 41 (04) : 770 - 777
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] Immobilised enzymes: science or art?
Cao, LQ
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2005, 9 (02) : 217 - 226
[5] Ch Chen J, 2010, CHINESE J CATAL, V31, P827
[6] Analysis of the interaction of lipases with polypropylene of different structure and polypropylene-modified glass surface
Foresti, M. L.
Ferreira, M. L.
[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2007, 294 (1-3) : 147 - 155
[7] Hu Y, 2010, PROG CHEM, V22, P1656
[8] Effect of pore diffusional resistance on biocatalytic activity of Burkholderia cepacia lipase immobilized on SBA-15 hosts
Jaladi, Hemachand
Katiyar, Amit
Thiel, Stephen W.
Guliants, Vadim V.
Pinto, Neville G.
[J]. CHEMICAL ENGINEERING SCIENCE, 2009, 64 (07) : 1474 - 1479
[9] Trypsin immobilization on mesoporous silica with or without thiol functionalization
Jang, Soohyun
Kim, Dongjun
Choi, Jungsik
Row, Kyungho
Ahn, Whaseung
[J]. JOURNAL OF POROUS MATERIALS, 2006, 13 (3-4) : 385 - 391
[10] Influence of pore diameters on the immobilization of lipase in SBA-15
Kang, Yu
He, Jing
Guo, Xiaodan
Guo, Xin
Song, Zhihong
[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2007, 46 (13) : 4474 - 4479

← 1 2 3 →