Evidence for hydrogen-deuterium exchange in viral particles

被引:0
|
作者
Mao, JS [1 ]
Liu, ZY
Tang, CH
He, YH
Zhu, JH
Wang, CY
Chai, SA
Chen, YQ
Qian, W
机构
[1] Zhejiang Acad Med Sci, Hangzhou 310013, Peoples R China
[2] Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China
[3] Chinese Acad Geol Sci, Beijing 100037, Peoples R China
来源
CHINESE SCIENCE BULLETIN | 2004年 / 49卷 / 03期
关键词
gas-isotope-mass spectrometry; virus thermostability; heavy water (D2O); hydrogen-deuterium exchange; Japanese encephalitis virus; hepatitis A virus; viral RNA;
D O I
暂无
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Heavy water (D2O) could enhance thermostability of some viruses. However, the underlying mechanism is not clear. Here we report the development of a matrix-aided gas-isotope-ratio mass spectrometry method that allows direct determination of deuterium/hydrogen (D/H) ratio in D2O-treated Japanese encephalitis virus (JEV), hepatitis A virus (HAV) and RNA from D2O-treated HAV. The D/H ratio was expressed as deltaD(SMOW). Our experiments showed that deltaD(SMOW) values increased significantly in D2O-treated viral samples compared to normal controls, and increment in deltaD(SMOW) of D2O, treated viral samples was in a fine linear relationship with increment in amount of samples loaded in BSA matrix. Our experiments also indicated that increased deltaD(SMOW) of D2O-treated virus correlated well with its enhanced thermostability. The results suggested that hydrogen-deuterium exchange occurred in viral particles and its RNA structure as a result of D2O-treatment. Furthermore, such exchange could cause changes in viral phenotype, such as enhanced thermostability.
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页码:253 / 257
页数:5
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