Evidence for hydrogen-deuterium exchange in viral particles

被引:0
|
作者
MAO Jiangsen
Department of Chemistry
Chinese Academy of Geologic Sciences
机构
关键词
gas-isotope-mass spectrometry; virus thermostability; heavy water (D2O); hydrogen-deuterium exchange; Japanese encephalitis virus; hepatitis A virus; viral RNA;
D O I
暂无
中图分类号
O611 [化学元素与无机化合物];
学科分类号
070301 ; 081704 ;
摘要
Heavy water (D2O) could enhance thermosta-bility of some viruses. However, the underlying mechanism is not clear. Here we report the development of a matrix-aided gas-isotope-ratio mass spectrometry method that allows direct determination of deuterium/hydrogen (D/H) ratio in D2O-treated Japanese encephalitis virus (JEV), hepatitis A virus (HAV) and RNA from D2O-treated HAV. The D/H ratio was expressed as δDSMOW.. Our experiments showed that δDSMOW values increased significantly in D2O-treated viral samples compared to normal controls, and increment in δDSMOW of D2O, treated viral samples was in a fine linear relationship with increment in amount of samples loaded in BSA matrix. Our experiments also indicated that increased δDSMOW of D2O-treated virus correlated well with its enhanced thermostability. The results suggested that hydrogen-deuterium exchange occurred in viral particles and its RNA structure as a result of D2O-treatment. Furthermore, such exchange could cause changes in viral phenotype,
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页码:253 / 257
页数:5
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