Nuclear accumulation of the U1 snRNP-specific protein C is due to diffusion and retention in the nucleus

被引：7

作者：

Gunnewiek, JMTK ^{[1
]}

vanAarssen, Y ^{[1
]}

vanderKemp, A ^{[1
]}

Nelissen, R ^{[1
]}

Pruijn, GJM ^{[1
]}

vanVenrooij, WJ ^{[1
]}

机构：

[1] UNIV NIJMEGEN, DEPT BIOCHEM, NL-6500 HB NIJMEGEN, NETHERLANDS

来源：

EXPERIMENTAL CELL RESEARCH | 1997年 / 235卷 / 01期

关键词：

D O I：

10.1006/excr.1997.3663

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The U1 small nuclear ribonucleoprotein particle (snRNP) has an important function in the early formation of the spliceosome, the multicomponent complex in which pre-mRNA splicing takes place. The nuclear localization signals of two of the three U1 snRNP-specific proteins, U1-70K and U1A, have been mapped. Both proteins are transported actively to the nucleus. Here we show by microinjection of Xenopus Laevis oo cytes that the third U1 snRNP-specific protein, U1C, passively enters the nucleus. Furthermore, we show that in both X. laevis oocytes and cultured HeLa cells mutant U1C proteins that are not able to bind to the U1 snRNP do not accumulate in the nucleus, indicating that nuclear accumulation of U1C is due to incorporation of the protein into the U1 snRNP. (C) 1997 Academic Press.

引用

页码：265 / 273

页数：9

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