Next-generation sequencing of cancer consensus genes in lymphoma

被引：10

作者：

Huellein, Jennifer ^{[1
,2
]}

Jethwa, Alexander ^{[1
,2
]}

Stolz, Tatjana ^{[1
,2
]}

Blume, Carolin ^{[1
,2
]}

Sellner, Leopold ^{[1
,2
,3
]}

Sill, Martin ^{[5
]}

Langer, Christian ^{[6
]}

Jauch, Anna ^{[4
]}

Paruzynski, Anna ^{[1
,2
]}

von Kalle, Christof ^{[1
,2
]}

Schmidt, Manfred ^{[1
,2
]}

Glimm, Hanno ^{[1
,2
]}

Zenz, Thorsten ^{[1
,2
,3
]}

机构：

[1] Natl Ctr Tumor Dis NCT, Dept Translat Oncol, D-69120 Heidelberg, Germany

[2] German Canc Res Ctr, D-69120 Heidelberg, Germany

[3] Univ Heidelberg Hosp, Dept Med 5, Heidelberg, Germany

[4] Univ Heidelberg Hosp, Inst Human Genet, Heidelberg, Germany

[5] German Canc Res Ctr, Div Biostat, D-69120 Heidelberg, Germany

[6] Univ Hosp Ulm, Dept Internal Med 3, Ulm, Germany

来源：

LEUKEMIA & LYMPHOMA | 2013年 / 54卷 / 08期

关键词：

Targeted resequencing; cancer consensus genes; lymphoma; chronic lymphocytic leukemia; CLL; multiple myeloma; CHRONIC LYMPHOCYTIC-LEUKEMIA; MUTATION; INACTIVATION; INHIBITION;

D O I：

10.3109/10428194.2013.796053

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Sensitive identification of mutations in genes related to the pathogenesis of cancer is a prerequisite for risk-stratified therapies. Next-generation sequencing (NGS) in lymphoma has revealed genetic heterogeneity which makes clinical translation challenging. We established a 454-based targeted resequencing platform for robust high-throughput sequencing from limited material of patients with lymphoma. Hotspot mutations in the most frequently mutated cancer consensus genes were amplified in a two-step multiplex-polymerase chain reation (PCR) which was optimized for homogeneous coverage of all regions of interest. We show that targeted resequencing based on NGS technologies allows highly sensitive detection of mutations and assessment of clone size. The application of this or similar techniques will help the development of genotype-specific treatment approaches in lymphoma.

引用

页码：1831 / 1835

页数：5

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