Line-Broadening in Low-Temperature Solid-State NMR Spectra of Fibrils

被引：26

作者：

Bauer, Thomas ^{[1
]}

Dotta, Claudio ^{[1
]}

Balacescu, Livia ^{[1
]}

Gath, Julia ^{[1
]}

Hunkeler, Andreas ^{[1
]}

Bockmann, Anja ^{[2
]}

Meier, Beat H. ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Phys Chem, Vladimir Prelog Weg 2, CH-8093 Zurich, Switzerland

[2] Univ Lyon 1, CNRS, UMR 5086, Inst Biol & Chim Prot, 7 Passage Vercors, F-69367 Lyon, France

来源：

JOURNAL OF BIOMOLECULAR NMR | 2017年 / 67卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

Solid-state NMR; Low-temperature; Line broadening; Fibrils; Protein; HET-s; DYNAMIC NUCLEAR-POLARIZATION; HET-S PRION; HET-S(218-289) AMYLOID FIBRILS; ATOMIC-RESOLUTION STRUCTURE; ANTIFREEZE PROTEIN; PODOSPORA-ANSERINA; MAS NMR; SPECTROSCOPY; SCHEME; ICE;

D O I：

10.1007/s10858-016-0083-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The temperature-dependent resonance-line broadening of HET-s(218-289) in its amyloid form is investigated in the range between 110 K and 280 K. Significant differences are observed between residues in the structured hydrophobic triangular core, which are broadened the least and can be detected down to 100 K, and in the solvent-exposed parts, which are broadened the most and often disappear from the observed spectrum around 200 K. Below the freezing of the bulk water, around 273 K, the protein fibrils are still surrounded by a layer of mobile water whose thickness decreases with temperature, leading to drying out of the fibrils.

引用

页码：51 / 61

页数：11

共 50 条

[31] LOW-TEMPERATURE SOLID-STATE C-13 NMR-STUDIES OF THE RETINAL CHROMOPHORE IN RHODOPSIN
SMITH, SO
PALINGS, I
COPIE, V
RALEIGH, DP
COURTIN, J
PARDOEN, JA
LUGTENBURG, J
MATHIES, RA
GRIFFIN, RG
BIOCHEMISTRY, 1987, 26 (06) : 1606 - 1611
[32] Revealing Electrolytic Ion Sorption in Layered Graphene Galleries through Low-Temperature Solid-State NMR
Lee, Daniel
Banda, Harish
Perie, Sandy
Marcucci, Coralie
Chenavier, Yves
Dubois, Lionel
Taberna, Pierre-Louis
Simon, Patrice
De Paepe, Gael
Duclairoir, Florence
CHEMISTRY OF MATERIALS, 2023, 35 (10) : 3841 - 3848
[33] LOW-TEMPERATURE SOLID-STATE C-13 NMR-STUDIES OF THE RETINAL CHROMOPHORE IN RHODOPSIN
SMITH, SO
PALINGS, I
COPIE, V
RALEIGH, DP
COURTIN, J
PARDOEN, JA
LUGTENBURG, J
MATHIES, RA
GRIFFIN, RG
BIOPHYSICAL JOURNAL, 1987, 51 (02) : A15 - A15
[34] A Strategy for Probing the Evolution of Crystallization Processes by Low-Temperature Solid-State NMR and Dynamic Nuclear Polarization
Vioglio, Paolo Cerreia
Thureau, Pierre
Juramy, Marie
Ziarelli, Fabio
Viel, Stephane
Williams, P. Andrew
Hughes, Colan E.
Harris, Kenneth D. M.
Mollica, Giulia
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2019, 10 (07): : 1505 - 1510
[35] Removal of Homogeneous Broadening from 1H-Detected Multidimensional Solid-State NMR Spectra
Perras, Frederic A.
Wang, Songlin
Mayer, Jacob
Halder, Mita
Paterson, Alexander L.
Culver, Damien B.
Rienstra, Chad M.
ANALYTICAL CHEMISTRY, 2025, 97 (08) : 4653 - 4660
[36] Molecular structure of amyloid fibrils: insights from solid-state NMR
Tycko, Robert
QUARTERLY REVIEWS OF BIOPHYSICS, 2006, 39 (01) : 1 - 55
[37] Low-temperature synthesis of uranium tetraboride by solid-state metathesis reactions
Lupinetti, AJ
Fife, JL
Garcia, E
Dorhout, PK
Abney, KD
INORGANIC CHEMISTRY, 2002, 41 (09) : 2316 - 2318
[38] LOW-TEMPERATURE DISPROPORTIONATION AND COMBINATION REACTIONS OF ALKYL RADICALS IN THE SOLID-STATE
TILQUIN, B
BULLETIN DES SOCIETES CHIMIQUES BELGES, 1982, 91 (05): : 462 - 462
[39] SOVIET-FRENCH SYMPOSIUM ON LOW-TEMPERATURE SOLID-STATE PHYSICS
ASLAMAZO.LG
VESTNIK AKADEMII NAUK SSSR, 1972, (06) : 94 - &
[40] Low-temperature solid-state NMR spectroscopy. A strategy for the direct observation of quadrupolar nuclides of biological interest
Ellis, Paul D.
Lipton, Andrew S.
ANNUAL REPORTS ON NMR SPECTROSCOPY, VOL 60, 2007, 60 : 1 - 38

← 1 2 3 4 5 →