CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms

被引：15
|
作者：
Huang, Zhanchao [1 ]
Fang, Jianhua [1 ]
Zhou, Min [2 ]
Gong, Zhenghua [2 ]
Xiang, Tianxin [1 ]
机构：
[1] Nanchang Univ, Med Ctr Major Publ Hlth Events Jiangxi Prov, Affiliated Hosp 1, Nanchang, Peoples R China
[2] Jiangxi Zhongke Yanyuan Biotechnol Co Ltd, Nanchang, Peoples R China
来源：
FRONTIERS IN MICROBIOLOGY | 2022年 / 13卷
关键词：
CRISPR; Cas13; rapid detection; pathogenic; microorganisms; MEDIATED ISOTHERMAL AMPLIFICATION; NUCLEIC-ACID DETECTION; CAS SYSTEMS; DETECTION PLATFORM; WEST-AFRICA; EBOLA-VIRUS; RNA; DNA; CLASSIFICATION; EVOLUTION;
D O I：
10.3389/fmicb.2022.1011399
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA to produce a sensitive and specific method with high base resolution and thus to provide a novel tool for the rapid detection of microorganisms. The review aims to provide insights to spur further development by summarizing the characteristics of effectors of the CRISPR-Cas13 system and by describing the latest research into its application in the rapid detection of pathogenic microorganisms in combination with nucleic acid extraction, isothermal amplification, and product detection.
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页数：14
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