Purification and properties of chitinase from Arthrobacter sp NHB-10

被引：7
|
作者：
Okazaki, K [1 ]
Kawabata, T
Nakano, M
Hayakawa, S
机构：
[1] Kagawa Univ, Fac Agr, Dept Life Sci, Kagawa 7610795, Japan
[2] Kagawa Univ, Fac Agr, Dept Biochem & Food Sci, Kagawa 7610795, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 09期
关键词：
chitinase; Arthrobacter; chitin;
D O I：
10.1271/bbb.63.1644
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A chitinase was purified from the culture filtrate of nigeran-degrading Arthrobacter sp. NHB-10 by precipitation with ammonium sulfate and column chromatographies on DEAE-Sephadex A-50 and Superose 12. The final preparation was homogenous in polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was 30,000 and its isoelectric point was 6.8. The optimum pH and temperature for the enzyme activity were 5.0 and 45 degrees C, respectively. The enzyme was stable from pH 3 to 7 and up to 55 degrees C. The enzyme activity was inhibited by Hg2+ and p-chloromercuribenzoic acid. Two internal amino acid sequences of the enzyme were AGPQLLTGW and IGGVMT.
引用
收藏
页码：1644 / 1646
页数：3
相关论文
共 50 条
  • [41] Purification and properties of extracellular phytase from Bacillus sp KHU-10
    Choi, YM
    Suh, HJ
    Kim, JM
    [J]. JOURNAL OF PROTEIN CHEMISTRY, 2001, 20 (04): : 287 - 292
  • [42] Purification and Characterization of Chitinase from Paenibacillus sp. D1
    Anil Kumar Singh
    Hari S. Chhatpar
    [J]. Applied Biochemistry and Biotechnology, 2011, 164 : 77 - 88
  • [43] PURIFICATION AND SOME PROPERTIES OF CHITINASE FROM ASPERGILLUS-CARNEUS
    ABDELNABY, MA
    ELSHAYEB, NMA
    SHERIEF, AA
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1992, 37 (02) : 141 - 154
  • [44] PURIFICATION AND PROPERTIES OF A CHITINASE FROM PENICILLIUM-OXALICUM AUTOLYSATES
    RODRIGUEZ, J
    COPAPATINO, JL
    PEREZLEBLIC, MI
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1995, 20 (01) : 46 - 49
  • [45] Arthrobacter gangotriensis sp nov and Arthrobacter kerguelensis sp nov from Antarctica
    Gupta, P
    Reddy, GSN
    Delille, D
    Shivaji, S
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2004, 54 : 2375 - 2378
  • [46] PURIFICATION AND CHARACTERIZATION OF HALOALCOHOL DEHALOGENASE FROM ARTHROBACTER SP STRAIN AD2
    VANDENWIJNGAARD, AJ
    REUVEKAMP, PTW
    JANSSEN, DB
    [J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (01) : 124 - 129
  • [47] PURIFICATION AND CHARACTERIZATION OF MALEATE HYDRATASE FROM ARTHROBACTER SP STRAIN MCI2612
    UEDA, M
    ASANO, Y
    YAMADA, H
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (09) : 1545 - 1548
  • [48] SARCOSINE OXIDASE FROM ARTHROBACTER-UREAFACIENS - PURIFICATION AND SOME PROPERTIES
    OGUSHI, S
    NAGAO, K
    EMI, S
    ANDO, M
    TSURU, D
    [J]. CHEMICAL & PHARMACEUTICAL BULLETIN, 1988, 36 (04) : 1445 - 1450
  • [49] PURIFICATION AND PROPERTIES OF LYTIC BETA-GLUCANASE FROM AN ARTHROBACTER BACTERIUM
    DOI, K
    DOI, A
    FUKUI, T
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1973, 37 (07): : 1619 - 1627
  • [50] Purification and properties of phenylethylamine oxidase of Arthrobacter globiformis
    Shimizu, E
    Ohta, K
    Takayama, S
    Kitagaki, Y
    Tanizawa, K
    Yorifuji, T
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (03) : 501 - 505
12345