Label-free cell profiling

被引：20

作者：

Schasfoort, Richard B. M. ^{[1
,2
]}

Bentlage, Arthur E. H. ^{[3
]}

Stojanovic, Ivan ^{[1
]}

van der Kooi, Alex ^{[2
]}

van der Schoot, Ellen ^{[3
]}

Terstappen, Leon W. M. M. ^{[1
]}

Vidarsson, Gestur ^{[3
]}

机构：

[1] Univ Twente, MIRA Inst, Med Cell Biophys Grp, NL-7522 NB Enschede, Netherlands

[2] IBIS Technol, NL-7521 PR Enschede, Netherlands

[3] Univ Amsterdam, Acad Med Ctr, Dept Expt Immunohematol, Sanquin Res & Landsteiner Lab, NL-1105 AZ Amsterdam, Netherlands

来源：

ANALYTICAL BIOCHEMISTRY | 2013年 / 439卷 / 01期

关键词：

Label free; Cell interaction analysis; Red blood cell; SPR imaging; Multiplex fingerprint; SURFACE-PLASMON RESONANCE;

D O I：

10.1016/j.ab.2013.04.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：4 / 6

页数：3

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